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Image Search Results
Journal: Cell reports
Article Title: linc-ADAIN , a human adipose lincRNA, regulates adipogenesis by modulating KLF5 and IL-8 mRNA stability
doi: 10.1016/j.celrep.2024.114240
Figure Lengend Snippet: (A) Regulatory features at the linc-ADAIN locus, RNA-seq coverage (human adipocytes), transcription factor binding, and active histone modification markers. (B) Tissue expression of linc-ADAIN from GTEx (gene mean transcripts per million [TPM]). (C and D) Induction of linc-ADAIN by the PPARγ agonist rosiglitazone (10 μM) (C) and during adipocyte differentiation in vitro (D) ( N = 3 in triplicate). (E) Cellular fractionation of primary ASC adipocytes. qPCR of MALAT1, GAPDH, and linc-ADAIN of nuclear and cytoplasmic fractions. Data were normalized by averaging of loading controls GAPDH, β-ACTIN, MALAT1, U6, and HPRT and then subtracting the nucleus value and getting a fold change of gene expression compared to nucleus ( N = 3). (F) RNA scope assay showing the spatial expression of linc-ADAIN (red) and nuclei (DAPI/blue) in scramble and linc-ADAIN shRNA hTERT ASC adipocytes (scale bar, 20 μm). (G) UMAP (uniform manifold approximation and projection) projection of linc-ADAIN (linc01230) expression in single-cell RNA-seq of human subcutaneous WAT (Broad Institute ). Data are presented as the mean ± SEM.
Article Snippet: Single cell UMAP data for
Techniques: RNA Sequencing, Binding Assay, Modification, Expressing, In Vitro, Cell Fractionation, Gene Expression, RNAscope, shRNA